Figure 5.

Broad Substrate Specificity of Cr-PDAT as a Lipase.

(A) Phospholipase activities toward various PLs. FFA, free fatty acid.

(B) Neutral lipid hydrolase activities toward TAG, DAG, and MAG.

(C) Galactolipase activities toward MGDG and DGDG.

(D) Steryl ester hydrolase activities of and toward cholesteryl ester (CSO). ORFCrPDAT (+*) and/or ΔTMCrPDAT (+) were used for enzymatic assays. Reactions without the addition of enzyme (−) were used to show the nonenzymatic hydrolysis of lipid substrates in the aqueous buffer. Free fatty acids (FFA) resulting from the hydrolysis of lipid substrates (S) are marked. Negative control (heat-inactivated PDAT) and positive control (commercial lipase) are shown in Supplemental Figure 12 online.