Figure 7.

Regulation of PDAT in C. reinhardtii (Wild Type).

(A) Expression profile of the Cr-PDAT gene under LL/nitrogen-replete (LL+N, circles), LL/nitrogen-depleted (LL-N, triangles), and HL/nitrogen-depleted (HL-N, squares) conditions. Gene expression was normalized to the expression of the 18 S rRNA gene. Data are presented as means ± se (n = 4).

(B) Immunoblotting analysis of protein level of Cr-PDAT in response to nitrogen deprivation stress. Cells grown in TAP medium under low light for 4 d were transferred to TAP-N medium. Protein concentration was determined using Image J software and normalized to the control (0 h, before induction).