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. 2012 Sep 25;24(9):3708–3724. doi: 10.1105/tpc.112.100701

Figure 9.

Figure 9.

Generation of amiRNA Knockdown Mutants.

(A) Knockdown of Cr-PDAT in P13i and P14i compared with the control transformed with an empty vector (TC), confirmed with real-time PCR.

(B) Immunoblotting analysis of protein level in the knockdown mutants and the control (TC). Protein concentration was determined using Image J software and normalized to the control.

(C) Growth kinetics of the knockdown mutants (P13i and P14i) and the control (TC) under N-replete conditions. Data are presented as means ± se (n = 4 to 6), with the asterisk indicating P < 0.05.