Figure 8.

Density of the Actin Array in cpb-1 Hypocotyl Epidermal Cells Is Insensitive to 1-Butanol Treatment.

Percentage of occupancy or density was measured and binned into three regions along the axial gradient of cell expansion in 5-d-old etiolated wild-type (WT) (A) or cpb-1 (B) hypocotyls after incubation with 0, 0.1, 0.5, or 1% 1-butanol or with 1% 2-butanol for 15 min. Wild-type seedlings treated with 1-butanol have significantly decreased filament density in the top, middle, and bottom regions of hypocotyls, and this reduction is dose dependent (A). By contrast, 1-butanol treatments of cpb-1 seedlings did not decrease the density (B). Treatments with 2-butanol had no measurable effect on actin filament levels in either wild-type or cpb-1 seedlings. Values given are means ± se (n = 300 images from 150 cells per region; 10 hypocotyls were measured for each treatment per genotype; *P < 0.05 and **P < 0.001, analysis of variance).