Table 1. Actin Dynamics Parameters for cpb-1 and Wild-type Epidermal Cells.
Stochastic Dynamics Parameters | Wild Type | cpb-1 |
---|---|---|
Elongation rate; µm/s | 1.64 ± 0.45 | 1.67 ± 0.54ND |
Severing frequency; breaks/µm/s | 0.012 ± 0.006 | 0.009 ± 0.005* |
Max. filament length; µm | 11.9 ± 3.5 | 15.0 ± 5.0* |
Max. filament lifetime; s | 21.4 ± 7.1 | 26.5 ± 9.8* |
Regrowth of severed ends; % | 2.9 ± 1.4 | 3.5 ± 1.4ND |
Annealing of severed ends; % | 2.1 ± 1.0 | 9.3 ± 3.0* |
Filament origin; % per cell | ||
de novo | 27.9 ± 12.7 | 27.6 ± 15.9 |
ends | 21.8 ± 8.7 | 28.2 ± 11.3† |
side | 50.3 ± 14.7 | 44.2 ± 15.4 |
Measurements taken from epidermal cells in the top third of 5-d-old hypocotyls of the cpb-1 mutant and corresponding wild-type sibling plants. Values given are means ± se, with n > 50 filaments from n > 10 epidermal cells and at least 10 hypocotyls per line. For filament origin: n > 30 cells from at least 10 hypocotyls per line. ND, not significantly different from the wild-type control value by Student’s t test; P value > 0.05. *Significantly different from the wild-type control value by Student’s t test; P value ≤ 0.05. †Significantly different from wild-type control group value by analysis of variance; P value < 0.0001.