Figure 2.

Deletion of hapX Affects Expression of Iron Regulatory Genes.

Quantitative real-time RT-PCR analysis was performed in the indicated strains germinated for 16 h in iron-depleted MM and transferred for 10 h to iron-depleted (−Fe) or iron-replete MM (+Fe). Transcript levels of hapX, sreA, and srbA genes are expressed relative to those of the wild-type (wt) strain grown under iron-replete conditions. Bars represent se from three independent experiments with three technical replicates each. Values with the same letter are not significantly different according to the Mann-Whitney test (P = 0.05).

[See online article for color version of this figure.]