Figure 7.

HapX Governs Virulence of F. oxysporum on Tomato Plants.

(A) Groups of 10 tomato plants (cv Monika) were inoculated by dipping roots into a suspension of 5 × 10⁶ or 10⁶ freshly obtained microconidia mL⁻¹ of the indicated fungal strains. Percentage survival was plotted for 30 d. Data shown are from one representative experiment. wt, wild type.

(B) Quantitative real-time RT-PCR analysis was performed with total RNA obtained from the wild-type strain grown on MM with or without iron as described in Figure 2 or from inoculated tomato roots 2, 5, and 10 DAI. Transcript levels are expressed relative to those in iron-replete conditions (+Fe). Bars represent se from three independent experiments with three technical replicates each. Values with the same letter are not significantly different according to the Mann-Whitney test (P = 0.05).

[See online article for color version of this figure.]