Figure 8.
HapX Is Essential for Dissemination and Virulence of F. oxysporum in Immunodepressed Mice.
(A) Groups of 10 immunodepressed Oncins France 1 male mice were inoculated with 2 × 107 microconidia of the indicated strains by lateral tail vein injection. Percentage survival was plotted for 15 d. Data shown are from one representative experiment. wt, wild type.
(B) Ten randomly chosen surviving mice from each treatment were sacrificed 7 DAI, and homogenates obtained from the indicated organs were quantitatively cultured on PDA medium.
(C) Quantitative real-time RT-PCR analysis was performed with total RNA obtained from the wild-type strain germinated for 20 h (16 h at 28°C + 4 h at 37°C) in iron-depleted MM and then transferred for the indicated time periods (min) to fresh MM or whole human blood. Transcript levels are expressed relative to those in MM at time 0. Bars represent se from three independent experiments with three technical replicates each. Values with the same letter are not significantly different according to the Mann-Whitney test (P = 0.05).
[See online article for color version of this figure.]