Table 1. Iron Metabolism-Related Genes Repressed by HapX Orthologs under Iron Starvation.
Gene | Gene Product | Wild Type ± Fe, log2 | Wild Type/hapx −Fe, log2 | Af | Ca | Sp | Sc |
---|---|---|---|---|---|---|---|
FOXG_02862 | Copper-resistance protein Crd2 | 3.93 | −2.44 | x | |||
FOXG_06292 | 3-isopropylmalate dehydratase (Ile/Leu/Val biosynthesis) | 3.17 | −4.09 | x | x | x | x |
FOXG_15294 | Mycelial catalase Cat2 (heme) | 3.10 | −1.84 | ||||
FOXG_04395 | Dihydroxy acid dehydratase (Ile/Val biosynthesis) | 3.07 | −1.56 | x | x | x | x |
FOXG_04047 | Vacuolar iron transporter Ccc1 | 2.74 | −2.79 | x | x | x | |
FOXG_10653 | Pyridine nucleotide-disulphide oxidoreductase | 2.71 | −1.62 | x | x | ||
FOXG_05207 | Aconitate hydratase, mitochondrial | 2.54 | −2.06 | ||||
FOXG_10442 | Nitrite/sulfite reductase ferredoxin-like half domain | 2.49 | −2.37 | ||||
FOXG_04849 | Cytochrome P450 monooxygenase | 2.39 | −1.61 | ||||
FOXG_09278 | Succinate dehydrogenase iron-sulfur protein; TCA cycle | 2.37 | −1.21 | x | x | x | |
FOXG_11281 | NADH-dependent glutamate synthase (GLT1) | 2.32 | −2.22 | x | x | x | x |
FOXG_12892 | lysF, Aconitase family (aconitate hydratase, Lys biosynthesis)a | 2.30 | −1.94 | x | x | x | |
FOXG_12260 | Peroxidase; catalase/peroxidase HPI | 2.27 | −1.48 | ||||
FOXG_01544 | Succinate dehydrogenase, flavoprotein subunit; TCA cycle | 2.24 | −1.25 | x | x | x | |
FOXG_01159 | Homocitrate synthase (Lys biosynthesis) | 2.21 | −1.00 | x | |||
FOXG_09472 | Cytochrome P450 | 2.20 | −1.08 | ||||
FOXG_05118 | Acyl-CoA dehydrogenase | 1.84 | −1.05 | ||||
FOXG_03713 | acoA, Aconitase family (aconitate hydratase), TCA cyclea | 1.73 | −1.41 | x | x | x | x |
FOXG_08910 | Cytochrome P450 monooxygenase | 1.63 | −1.97 | ||||
FOXG_00142 | Cytochrome c peroxidase | 1.54 | −1.33 | x | x | ||
FOXG_06266 | Siderophore transcription factor SreAa | 1.23 | −1.60 | x | x | x | |
FOXG_06399 | FMN-dependent dehydrogenase | 1.17 | −2.22 | ||||
FOXG_12815 | cycA, Cytochrome ca | 1.12 | −1.49 | x | x | x | x |
FOXG_01103 | Hemerythrin HHE cation binding domain-containing protein | 1.06 | −1.22 |
F. oxysporum genes that fulfill the following criteria in microarray-based transcriptional profiling: (1) More than twofold downregulated in the wild type under steady state iron starvation versus iron sufficiency (Wild Type ± Fe) and (2) more than twofold upregulated during steady state iron-starved growth in ΔhapX compared with the wild type (Wild Type/hapx − Fe). Genes are ranked based on the level of upregulation in the Wild Type ± Fe condition. The x indicates genes previously identified to be similarly regulated by the HapX orthologs in A. fumigatus (Af; Schrettl et al., 2010), C. albicans (Ca; Chen et al., 2011), and S. pombe (Sp; Mercier et al., 2008) or by Cth1 and Cth2 in S. cerevisiae (Sc; Puig et al., 2008).
Genes whose differential expression was confirmed separately by real-time RT-PCR.