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. 2012 Sep 28;24(9):3823–3837. doi: 10.1105/tpc.112.103374

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© 2012 American Society of Plant Biologists. All rights reserved.

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Figure 4. — Expression of the Chimeric Suppressor VND7-SRDX Reduces the Transdifferentiation Rate in Arabidopsis.

Transgenic Arabidopsis (Col-0) expressing VND7-SRDX under the control of the Verticillium-induced PER21 promoter were generated and analyzed for Verticillium-induced transdifferentiation. Wild-type VND7 and VND7-SRDX expression were determined in transgenic plants.

(A) Real-time PCR analysis of VND7 and VND7-SRDX expression at 17 DAI in noninfected control and infected transgenic VND7-SRDX expressor plant L18.9; data represent means ± sd (n = 3 plants per time point).

(B) Phenotypes of wild-type Col-0 and the representative transgenic PER21_pro:VND7-SRDX line L18.9 under control conditions and upon Verticillium infection. Epifluorescence images of corresponding leaf veins are shown (Bottom); arrowheads designate de novo–formed TEs. dpi, days postinoculation.

(C) Percentage of transdifferentiated bundle sheath cells in infected transgenic VND7-SRDX expressor lines compared with infected wild-type Col-0 plants. The number of transdifferentiated bundle sheath cells per millimeter of leaf vein was determined microscopically, and results from wild-type plants were set to 100%. The degree of transdifferentiation in transgenic lines is shown as a percentage of transdifferentiation in wild-type plants. Data represent means ± sd (n = 5 plants, leaves 7 to 10 of each plant were analyzed, and the experiment was repeated twice). Significant differences between wild-type Col-0 and transgenic PER21_pro:VND7-SRDX lines at P ≤ 0.001 are indicated by ***.

(D) The percentage of protoxylem (pxl) and metaxylem (mxl) cells in infected transgenic VND7-SRDX expressor and wild-type Col-0 plants. Total number of transdifferentiated cells in wild-type and transgenic plants was set to 100%. Data represent means ± sd (n = 5 plants, 50 cells in leaves 7 to 10 of each plant were analyzed, and the experiment was repeated twice). Significant differences between wild-type Col-0 and transgenic PER21_pro:VND7-SRDX lines at P ≤ 0.05, P ≤ 0.01 and P ≤ 0.001 are indicated by *, **, and ***.

Bars in (B) = 1 cm for photographs and 25 μm for micrographs.