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. 2012 Sep 28;24(9):3823–3837. doi: 10.1105/tpc.112.103374

Figure 6.

Figure 6.

Analyses of Drought Stress Reporter Lines and Drought Stress Marker Genes Reveal Reduced Responsiveness of V. longisporum Strain VL43–Infected Plants.

(A) Noninfected control and infected RD29Bpro:GUS plants were exposed to drought stress (watering stopped at 7 DAI). Histochemical GUS staining of leaves was done at 21 DAI; the experiment was repeated three times, and representative data are shown.

(B) Noninfected control and infected ATHB6pro:LUC plants were exposed to drought stress (watering stopped at 7 DAI). Luciferase activity in leaves was monitored at 21 DAI (left). Note the lower luciferase activity in infected drought-stressed plants compared with noninfected plants. Macroscopic plant phenotypes are shown on the right. The experiment was repeated two times with similar results. dpi, days postinoculation.

Real-time PCR expression analysis of RD29B (C), ANAC072 (D), DREB2A (E), and PLDδ (F) was performed in noninfected control and infected transgenic Col-0 plants at 17 DAI; data represent means ± sd (n = 3 plants per time point).