Figure 1.

RRBSs in the Promoter of the RR4 and NSP2 Nodulation-Related Genes.

(A) Consensus sequence bound by the RR1 BD identified by SELEX. Numbers on the x and y axes, respectively, represent nucleotide positions and the frequency of occurrence (shown by letter size normalized from 0 to 1) of each nucleotide for each position (WebLogo; Crooks et al., 2004).

(B) and (C) Schematic diagram of RR4 (B) and NSP2 (C) promoter regions. The RRBS cis-element variants (boxes 1 to 6) are indicated. Alignment to the major SELEX consensus is shown below each promoter diagram. Gray indicates nonconserved nucleotides, underlined indicates conserved nucleotides between all boxes of each promoter, italics indicates 8-bp RRBS core, and st + or str − indicates DNA strand of sequences shown.