Figure 4. 15dPGJ₂ inhibition of LXR target genes mRNA expression is mediated by oxidative stress in human neutrophils.

Neutrophils were preincubated at 37°C with or without 10 µM DEM, 100 µM TEMPO (A), 100 µM vitamin E analog (Vit E) or 1 mM GSH (B) for 1 h. Then the cells were treated or not with 10 µM 15dPGJ₂ for 1 h, and thereafter stimulated or not with 1 µM T0901317 for 18 h. Finally, the levels of LXRα target genes mRNAs were analyzed by real time RT-PCR. Statistical data (mean ± SEM, n = 3) were corrected for differences in β-actin mRNA levels and expressed as fold induction. * P<0.01 for T0901317–stimulated, 15dPGJ₂ (A and B) or DEM (A)-treated versus -untreated. ^◊ P<0.01 for 15dPGJ₂ and T0901317-stimulated, TEMPO (A), Vit E or GSH (B)-treated versus -untreated.