Figure 7. 15dPGJ2 promotes LXRα serine phosphorylation in human neutrophils.
Neutrophils were preincubated at 37°C with or without 30 µM PD098059 for 30 min. Then the cells were treated or not with 10 µM 15dPGJ2 for 30 min, and thereafter stimulated or not with 1 µM T0901317 for further 30 min. Finally, the cells were lysed, LXRα was immunoprecipitated and Western blotting analysis of phosphoserine (P-Ser) and LXRα levels was carried out as indicated in Experimental Procedures. P-Ser levels are given in arbitrary units. The blot is representative of a set of three experiments yielding similar results.