Figure 2. EstA esterase is a target of the CbrAB/Crc cascade.

β-Galactosidase activities of an estA’-‘lacZ fusion were measured in PAO1 (black diamonds), PAO1ΔcbrB (black triangles), PAO1ΔcrcZ (black squares) and PAO1Δcrc (black circles) harbouring a plasmid with a translational estA’-‘lacZ fusion (pTLestA) (A) in LB medium and (B) in BSM medium supplemented with 40 mM succinate. (C) β-Galactosidase activities derived from a translational estA’-‘lacZ fusion with a mutated CA motif (pTLestA-ΔCA) were determined in PAO1 (black diamonds), PAO1ΔcbrB (black triangles), PAO1ΔcrcZ (black squares) and PAO1Δcrc (black circles). The strains were grown in BSM supplemented with 40 mM succinate. Cell growth was monitored by measuring the optical density at 600 nm (OD₆₀₀) (white symbols).