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. 2012 Oct 24;7(10):e44637. doi: 10.1371/journal.pone.0044637

Figure 3. Growth on acetate and acsA expression are under CbrAB/Crc control.

Figure 3

(A) Growth of PAO1 (black diamonds), PAO1ΔcbrB (black triangles), PAO1ΔcrcZ (white squares) and PAO1Δcrc (white circles) was measured in BSM supplemented with 40 mM acetate. (B) β-Galactosidase expression of a translational acsA’-‘lacZ fusion (pME10044) was monitored in PAO1 (black diamonds), PAO1ΔcbrB (black triangles), PAO1ΔcrcZ (black squares) and PAO1Δcrc (black circles). (C) Expression of a translational acsA’-‘lacZ fusion where the CA motif had been mutated (pME10045) was followed in PAO1 (black diamonds) and PAO1Δcrc (black circles). Cells were grown in BSM supplemented with 5 mM succinate and 40 mM acetate. The corresponding growth curves are shown in white symbols.