Figure 1. Differential production of ACT in B. bronchiseptica strains grown in 5% CO₂.

Strains RB50 (A, C) and 761 (B, D), grown in normal atmospheric oxygen conditions (A,B) or in elevated 5% CO₂ conditions (C, D). (E) RB50 and 761 grown in atmospheric conditions or grown in 5% CO₂ conditions, or recombinant ACT (2.5 ng) were probed with a monoclonal antibody to CyaA protein at a dilution of 1∶1000. J774 murine macrophage cells were stimulated with media or media containing RB50 (F) or 761 (G) at an MOI of 1 for 30 minutes, and cAMP levels were assessed. * indicates a p-value less than 0.05.