Figure 5. Differential transcription of 5% CO₂ responsive genes independent of bvgS expression.

qRT-PCR analysis was performed on RB50 grown in 5% CO₂ (light grey bars), RB54 in ambient air (dark gray bars), and RB54 in 5% CO₂ (black bars) compared to RB50 grown in ambient air (white bars). Fold-change expression (FCE) in all strains was expressed as mean ± standard deviation for cyaA (A), fhaB (B), bopD (C), bopB (D), cheZ (E), and flgB (F). Data shown are averages obtained from quadruplicate cultures. * indicates a p-value less than 0.05.