Skip to main content
. 2012 Oct 24;7(10):e47709. doi: 10.1371/journal.pone.0047709

Figure 2. Analysis of DNA methylation status of miR-200f promoter sequences in breast tumors.

Figure 2

(A) Schematic depiction of miR-200b-a-429 and miR-200c-141 genomic loci showing CpG islands (green), putative transcription start sites (TSS) and miRNA stem-loop sequences (red). The regions analyzed for DNA methylation (MassArray) are indicated by a black bar. Chromosomal location is indicated between brackets. (B) The DNA methylation levels across the regions shown in panel (A) were quantified in breast cancers by Sequenom MassArray® MALDI-TOF platform. The mean percentage of methylation levels in three tumor types (ER+, TN, MBC) are represented as box-plots. Statistical significance was determined by Student’s t test. (C) Expression and %CpG methylation data are represented on a scatter plot matrix showing correlation between the indicated variables, a 95% bivariate normal density ellipse is imposed on each scatterplot. Pearson correlation coefficients (R) and significance probabilities (P) are shown. Statistically significant p values (<0.05) are highlighted in red.