Figure 1. Substrate spectrum of LipS and LipT shown as relative activity on 4-nitrophenyl (pNP) esters with fatty acid chains of 4 to 18 C-atoms.

Reactions were incubated at 70°C (LipS) or 75°C (LipT) with final substrate concentrations of 1 mM in potassium phosphate buffer (PB, 0.1 M, pH 8.0). Extinction was measured at 405 nm against an enzyme-free blank. Data are mean values of at least three independent measurements and bars indicate the standard deviation.