Figure 2. Temperature optimum (A) and thermal stability (B) of LipS and LipT.

Data are mean values of at least three independent measurements and bars indicate the standard deviation. Temperature range and optimum of LipS and LipT were measured with pNP-dodecanoate at temperatures ranging from 20°C to 90°C for 10 min. Assays were performed by incubation of the enzymes at 70°C for up to 72 hours and by measuring residual activities with pNP-dodecanoate at 70°C (LipS) and 75°C (LipT).