Table 1. Biochemical parameters of recombinant LipT and LipS determined using 4-nitrophenol-decanoate (C10) for LipT and –octanoate (C8) for LipS.

Enzyme	U/mg	vmax (mol min−1)	Km (mol l−1)	kcat (min−1)	kcat/Km (M−1 sec−1)
LipT	0.6	5.4 • 10−8	1.1 • 10−3	0.1	0.8
LipS	12.0	2.0 • 10−7	2.2 • 10−3	1.3	10.3

The measurements were performed at 75 and 70°C, respectively, in 0.1 M PB pH 8.0.

Data are mean values of three independent measurements.