Table 2. Specific activity^* (U/mg) of LipT and LipS on pNP esters.

pNP-Substrate		LipT	LipS	CalB	ROL
Octanoate 1)	/	+	+ +	+	+
2-Phenylpropanoate 2)	rac	−	+ +	n. d.	n. d.
3-Phenylbutanoate 2)	rac	+	+	n. d.	n. d.
Cyclohexanoate 2)	/	−	+ + + +	n. d.	n. d.
2-(3-Benzoylphenyl) propanoate 2)	rac	+	+ +	n. d.	n. d.
2-Naphtoate 2)	/	+	+	n. d.	n. d.
1-Naphtoate 2)	/	+	−	n. d.	n. d.
Adamantanoate 2)	/	−	−	n. d.	n. d.
Methyldecanoate 1)	(S)	+	+	+	+
	(R)	+	+ +	+	+
2,3-Dihydro-1H-indene-1-carboxylate (indan acid ester) 1)	(S)	+	+	+	−
	rac	+	+	+	+
	(R)	+	+ +	+	+
Ibuprofen ester 1)	(S)	+	−	+	−
	rac	+	+ +	+	+
2-(4-isobutylphenyl)-N-(4-nitrophenyl) propanamide (Ibuprofen amide ester) 2)	rac	+	+	n. d.	n. d.
Naproxen ester 1)	(S)	+	+	+	+
	rac	+	+	+	+
	(R)	+	+ +	+	+

CalB (purchased from Sigma-Aldrich, Buchs, Switzerland) and ROL (purchased from Fluka/Sigma-Aldrich, Buchs, Switzerland) were used as references.

The extinction was measured spectrophotometrically against an enzyme-free blank with

¹⁾0.33 mM substrate solution (final concentration in 0.05 M Soerensen buffer pH 8.0 containing 0.1% gum arabic, 5 mM sodium deoxycholate and 10% DMSO) after incubation at 60°C (CalB) or 65°C (LipT, LipS, ROL) at 410 nm (ε = 7,392 M⁻¹ cm⁻¹).

²⁾0.5 mM substrate solution (final concentration in 0.1 M PB pH 8.0) after incubation at 70°C at 405 nm (ε = 19,454 M⁻¹ cm⁻¹).

^*Specific activity: n. d., not determined; −, no detectable activity or <0.01 U/mg; +, 0.01–0.30 U/mg; + +, 0.31–0.60 U/mg; + + +, 0.61–0.90 U/mg; + + + +, 0.91–1.26 U/mg. Specific activities of CalB and ROL refer to the dry-weight of the lyophilisate. Data are mean values of three independent measurements.