Figure 5. Suppression of SP1 and GATA1 expression leads to up-regulation of Ccna1 promoter activity.

The reporter constructs used for transfection are shown schematically on the left. Sp1 and GATA1 binding sites are marked with white ellipses and black diamonds, respectively. Reporter plasmids and corresponding siRNA oligonucleotides (100 µM) were co-transfected into NIH3T3 and GC-4spc cells. In all experiments pRL-CMV was co-transfected as an internal control. Bars show the average luciferase activity of five independent experiments, normalized with respect to the Renilla luciferase activity.