Figure 4. Effects of salinity on the antibacterial activity of Apl_avian β-defensins (Apl_AvBDs) against M. tetragenus or P. multocida.

(A) 25 µg/mL Apl_AvBDs; (B) 100 µg/mL Apl_AvBDs. Apl_AvBDs were incubated separately with either M. tetragenus or P. multocida for 3 h in the presence of 0, 50, 100, and 150 mM NaCl. All assays were performed in three independent experiments with three replicates per experiment, and each bar represents the mean ± SD. The data were analyzed using SAS [24].