Figure 6. Hemolytic activities of duck avian β-defensins (AvBDs).

Freshly isolated duck red blood cells were incubated with different concentrations of AvBDs (0–500 µg/mL). Release of hemoglobin, as a measure of hemolysis, was measured at 405 nm. Release of hemoglobin upon addition of 1% Triton X-100 was set at 100%. The percentage of hemolysis was calculated as [(A _{405 nm, peptide} – A _{405 nm, PBS})/(A _{405 nm, 1% Triton X-100}– A _{405 nm, PBS})] × 100%. All assays were performed in three independent experiments, with three replicates per experiment, and each point is the mean ± SD. The data were analyzed using SAS [24].