Table 1. PCR primer sequences and predicted product lengths.

Target mRNA	Sense primer (5′-3′)	Antisense primer (5′-3′)	Product size (bp)	GenBank accession no
Apl_ AvBD1 (RT-PCR)	AAACCATGCGGATCGTGTACCTGC	ATGGGGGTTGTTTCCAGGAGC	264	JQ359441
Apl_ AvBD3 (RT-PCR)	GAACTGCCACTCAGTGCAGAAT	ATGGGGGTTGTTTCCAGGAGC	183	-
Apl_ AvBD5 (RT-PCR)	ATGCAGATCCTGCCTCTCCTCTTTGCT	TCAGGAATACCATCGGCTCCGGCAGCAGAA	201	JF949720
Apl_ AvBD6 (RT-PCR)	ATGAGGATCCTTTACCTG	TCAGGCCCACCTGTTCCT	204	JQ359446
Apl_ AvBD16 (RT-PCR)	CCTTCTTCCTCTTGTTTCTCCAG	ATGGGGGTTGTTTCCAGGAGC	216	JQ359445
Duck β-actin (RT-PCR and real time PCR)	CCGTAAGGACCTGTACGCCAACAC	GCTGATCCACATCTGCTGGAAGG	208	AY251275
Apl_ AvBD1 (real time PCR)	GAAACAAGGAGAAATGTCATCG	ATGGGGGTTGTTTCCAGGAGC	183	JQ359441
Apl_ AvBD3 (real time PCR)	GAACTGCCACTCAGTGCAGAAT	ATGGGGGTTGTTTCCAGGAGC	183	–
Anser AvBD5 (real time PCR)	GCTGTCCCTTGCTCGAGGATT	GGAATACCATCGGCTCCGGC	139	JF949720
Apl_ AvBD6 (real time PCR)	GTCAGCCCTACTTTTCCAGC	GCCCACCTGTTCCTCACAC	143	JQ359446
Apl_ AvBD16 (real time PCR)	GAAACTCTGTCCTCTGCAGAAT	ATGGGGGTTGTTTCCAGGAGC	183	JQ359445
Apl_ AvBD1 (protein expression)	GAATTC ATGCGGATCGTGTACCT	GTCGAC TCAACCAAATATC	210	JQ359441
Apl_ AvBD3(protein expression)	GGATCC ATGACTGCCACTCAGTG	GTCGAC TCAATGGGGGTTGTTTC	198	–
Apl_ AvBD5 (protein expression)	GAATTC ATGCAGATCCTGCCTCTC	GTCGAC TCAGGAATACCATCGGCTCCGGCA	208	JF949720
Apl_ AvBD 6 (protein expression)	GAATTC ATGAGGATCCTTTACCTG	GTCGAC TCAGGCCCACCTGTTCCT	216	JQ359446
Apl_ AvBD16 (protein expression)	GGATCC ATGTTCTTCCTCTTGTTTCT	GTCGAC TCAACCCCATGTCCT	168	JQ359445