Figure 5. Formation of ternary receptor/ligand-phosphatase complexes.

Both the PYL1 and PYL2 receptors were able to interact with the HAB1 and ABI2 phosphatases to form complexes in the presence of (+)-ABA. Melt curves of ligand-bound receptors (dashed blue lines), PP2Cs (dashed red lines), and receptor-PP2C complexes (solid green lines). Receptors and PP2C were at 10 µM, (+)-ABA at 500 µM. (A) PYL1 with ABI2 (left) and HAB1 (right). (B) PYL2 with ABI2 (left) and HAB1 (right).