Skip to main content
. 2012 Oct 24;7(10):e47857. doi: 10.1371/journal.pone.0047857

Figure 9. SnRK2 kinase inhibitors affect the thermostability of the SnRK2.3 moiety, but not the MBP moiety, of a MBP-SnRK2.3 fusion protein.

Figure 9

(A) Melting profiles of SnRK2.3 (green), MBP (brown), and a MBP-SnR2.3 fusion protein (blue). The fusion protein underwent two separate unfolding transitions: the transition at lower temperature corresponds to that of the kinase while the unfolding at the higher temperature corresponds to that of the more stable MBP tag. (B–D) Melt curves of SnRK2.3, MBP, and the MBP-SnRK2.3 fusion protein in the presence of Wee1 inhibitor (B), staurosporine (C), and 500 mM NaCl (D). The color code is the same as in (A), with dashed lines representing the melt curves in the absence of inhibitors and solid lines those with inhibitors. Dotted black lines indicate the Tm of SnRK2.3 and MBP in the absence of inhibitors.