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. 2012 Oct 24;7(10):e47948. doi: 10.1371/journal.pone.0047948

Figure 4. Allopurinol prevents fructose-induced CHREBP acetylation and nuclear translocation in human hepatocytes.

Figure 4

A) KHK mRNA expression in cells exposed to fructose (5 mmol/L) for different time points. B) Analysis of acetylation state in immunoprecipitated ChREBP in cells exposed to glucose, fructose or mannitiol in the presence or absence of allopurinol. (C–E) ChREBP and KHK expression in nuclear and cytoplasmic extracts of cells control and incubated with glucose (25 mM) and fructose (5 mM) in the presence or absence of allopurinol.