Table 1. Quantification of hTERT mRNA variants by competitive RT-PCR.
Exons | Construct | Molecules per cell |
1–2 | INS-i1 | 2.5±2.2 |
Wildtype | 10±3.8 | |
1–3 | Wildtype | 17±6.0 |
DEL-e2 | 19±6.4 | |
2–4 | INS-i2[1–349] | 4.5±3.7 |
Wildtype | 18.5±15.6 | |
3–5 | Wildtype | 22±7.9 |
4–10 | Wildtype | 4.5±1.3 |
Alpha | 1.25±1.1 | |
Beta | 5.5±1.5 | |
Alpha-Beta | 0.65±0.5 | |
9–14 | INS-i13 | 1±0.3 |
Wildtype | 5±1.3 | |
13–16 | INS-i14 | 2±0.5 |
INS-i14[619–781]* | 4±1.0 | |
INS-i14[622–705]* | 3±1.6 | |
Wildtype | 5.5±1.5 |
Residue numbers inserted from intron 14 are indicated in brackets, with the first nucleotide of intron 14 denoted as “1”.