Figure 3. Comparison of wt T3D with jin-viruses in terms of plaque size, yield in U118MG cells and primary human fibroblasts (VH10 cells).

(A) Yield of wt T3D and jin-1, jin-2 and jin-3 mutants from U118MG cells infected with MOI 10 per virus. Yields were determined 72 hours post infection by plaque assays on 911 cells. The graph shows yields (Log₁₀ PFU) of two independent U118MG cell infections: first one is shown as a white bar and second as a grey bar. The dashed line represents the input amount of the initial infection. (B) Yield of wt T3D and jin-1, jin-2 and jin-3 mutants from VH10 cells infected with MOI 10 per virus. Yields were determined 72 hours post infection by plaque assays on 911 cells. The graph shows yields (Log₁₀ PFU) of two independent VH10 cell infections: first one is shown as a white bar and second as a grey bar. The dashed line represents the input amount of the initial infection. (C) Plaque sizes of wt T3D and jin-1, jin-2 and jin-3 mutants in 911 cells. Surface areas of 10 plaques per virus were measured four days post infection with Olympus DP-software.