Figure 6. Zip14^−/− mice exhibit altered zinc transporter expression and insulin and glucagon abundance in pancreas.

(A) Total RNA isolated from pancreas and Zip14, Zip10 and ZnT8 mRNAs were measured by qPCR and TBP was used as a normalizer. Immunofluoresence microscopic analysis showing ZIP14 abundance (B) and ZnT8, insulin colocalization (C) in pancreas of the WT and Zip14^−/− mice after LPS. (D) Immunohistochemical analysis of insulin and glucagon abundance in pancreatic islets of WT and Zip14^−/− mice that were administered LPS (2 mg/kg or saline; 0. 5 mL; i.p.) 18 hr before being killed. Values are mean ± SD, n = 5.