Figure 1.

Imipramine induces pMeCP2 in the GABAergic interneurons of the NAc. a, C57BL/6 mice were injected with imipramine (20 mg/kg, i.p.) in the home cage and then perfused at 0, 0.5, 2, 4, or 24 h after injection. pMeCP2 immunoreactivity in the NAc was quantified and averaged across mice at each time point. b, pMeCP2 induction after vehicle or 20 mg/kg imipramine (2 h after injection). Dashed white box was used as the inset image to examine pMeCP2 and GAD67 overlay in c. ac, Anterior commissure. c, pMeCP2 (red), GAD67 (green), and overlay immunostaining in the NAc 2 h after treatment with 20 mg/kg imipramine. White arrows indicate neurons coimmunolabeled with antibodies for pMeCP2 and GAD67. Scale bars, 20 μm. n = 4–5 mice per group. For a, *p < 0.05.