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A. In control experiments M3-R activation by BeCh causes a two phase contraction of the IAS; phasic followed by the tonic. SScIgG (but not NIgG and pooledhIgG) in resemblance with darifenacin (M3-R selective inhibitor) causes significant and concentration-dependent decrease in the BeCh-induced sustained contraction (*; p<0.05; n=6). B. Actual tracings of the effect of darifenacin, SScIgG, NIgG, and pooledhIgG on the BeCh-induced sustained contraction of the IAS.
