Figure 5. Hedgehog signaling controls metabolic reprogramming during liver injury in vivo.

Immunohistochemistry identifies cells expressing the M2 isozyme of PKM2, a specific marker of glycolytic activity, in healthy adult mice (A) and different liver injury models (B-D). Hh signaling was inhibited in PH-mice by cyclopamine (B), in aged MDR2^-/- mice by GDC-0449 (C), or abrogated selectively in MF by treating DTG-mice with tamoxifen (D), as indicated.