Fig. 3.

Effects of AM6701 and AM6702 on cellular integrity in the excitotoxic slice model. From the Fig. 2 treatment groups, slice cultures were treated with vehicle AM6701 or AM6702 for 1 h, and subsequently during the 2-h kainic acid (KA) exposure, as per the described protocol. The slices were fixed 24-h post-insult, then sectioned and stained with cresyl violet. Photomicrographs of field CA1 are shown and pyknotic nuclei are especially evident in the KA + vehicle group. DG = dentate gyrus; sp = stratum pyramidale; sr = stratum radiatum. Scale bar: 45 μm. Pyknotic nuclei were counted across multiple CA1 view fields of 8.5 × 10⁴ μm² per cultured slice, and mean counts ± SEM are shown in the lower graph (analysis of variance, p = 0.001). Post hoc test compared to KA only group: **p < 0.01