Fig. 8.

Assessment of pGluA1 in the kainic acid (KA) rat model. No-insult control rats from the Fig. 6 experiment, along with the KA groups treated with or without AM6701 or AM6702, were assessed for GluA1 phosphorylated at serine-845 (pGluA1-Ser-845) and actin in hippocampal homogenates (a). A nitrocellulose strip containing identical immunoblot samples was pre-treated with alkaline phosphatase (AP) at 37°C before incubating with the antibody against pGluA1-Ser-845, indicating phosphorylation-specific staining. Integrated optical densities for pGluA1-Ser-845 are shown as means ± SEM (b), indicating that AM6701 is more influential than AM6702 (analysis of variance, p < 0.01; n = 7–9). Post hoc tests compared to KA only group: *p < 0.05