Fig. 1.

Representative images of ZnT3⁺ neurons located in porcine jejunum. All images are composites of merged images taken separately from blue, red, and green fluorescent channels. a Myenteric plexus (MP); ZnT3 (red) neurons labeled for PGP9.5 (blue) but SP (green)-immunonegative (two single arrows). b Outer submucosal plexus (OSP); ZnT3 (red) neurons labeled for PGP9.5 (blue) but SP (green)-immunonegative (three single arrows). c Inner submucosal plexus (ISP); ZnT3 (red) neurons labeled for PGP9.5 (green) but SOM (blue)-immunonegative (two single arrows). d–i ZnT3⁺ neurons located in MP of porcine jejunum. d ZnT3 (red) neuron labeled for NOS (blue) and VAChT (green)-immunonegative (four single arrows), and ZnT3 (red) neurons labeled for VAChT (green) but NOS (blue)-immunonegative (two single small arrows). e ZnT3 (red) neurons labeled for VIP (blue) but VAChT (green)-immunonegative (two single arrows), and ZnT3 (red) neurons labeled for VAChT (green) but NOS (blue)-immunonegative (single small arrow). f ZnT3 (red) neuron labeled for VAChT (green) but SP (blue)-immunonegative (two single small arrows). g ZnT3 (red) neuron labeled for NOS (blue) but VAChT (green)-immunonegative (two single arrows). h ZnT3 (red) neurons labeled for SP (blue) but VIP (green)-immunonegative (single arrow), and ZnT3 (red) neurons labeled for VIP (green) but SP (blue)-immunonegative (single small arrow). i ZnT3 (red) neurons labeled for LENK (blue) but SOM (green)-immunonegative (single arrow), and ZnT3 (red) neurons labeled for SOM (green) but LENK (blue)-immunonegative (single small arrow). Bar 25 μm