Table 1.
Specification of immune reagents vs. zinc transporter 3 (PGP9.5 pan-neuronal marker, ZnT3 zinc transporter 3, NOS nitric oxide synthase, VIP vasoactive intestinal peptide, SP substance P, SOM somatostatin, LENK leu-enkephalin, VAChT vesicular acetylcholine transporter, NPY neuropeptide Y, GAL galanin, CGRP calcitonin-gene related peptide, FITC fluorescein isothiocyanate, AMCA 7-amino-4-methylcoumarin-3-acetic acid, H heavy chain, L light chain)
| Antisera | Code | Host species/specificity | Dilution | Supplier |
|---|---|---|---|---|
| Primary antibody | ||||
| PGP9.5 | 7863-2004 | Mouse | 1:2000 | Biogenesis, UK;www.biogenesis.co.uk |
| ZnT3 | – | Rabbit | 1:600 | Gift from Prof. Palmiter, USA |
| NOS | N2280 | Mouse | 1: 2000 | Sigma, US; www.sigma-aldrich.com |
| VIP | 9535-0504 | Mouse | 1: 2000 | Biogenesis |
| SP | 8450-0505 | Rat | 1:300 | |
| SOM | 8330-0009 | Rat | 1: 100 | |
| LENK | 4140-0355 | Mouse | 1: 1000 | |
| VAChT | H-V007 | Goat | 1: 2000 | Phoenix, Pharmaceuticals, US; www.phoenixpeptide.com |
| NPY | NZ1115 | Rat | 1:300 | Biomol Research Laboratories, US |
| GAL | T-5036 | Guinea pig | 1:1000 | Peninsula Labs, US; see Bachem; www.bachem.com |
| CGRP | T-5027 | Guinea pig | 1:1000 | |
| Secondary antibodies | ||||
| FITC-conjugated IgG (H + L) | 715-095-151 | Donkey-anti-mouse | 1:800 | Jackson |
| 712-095-153 | Donkey-anti-rat | 1:800 | ||
| 706-095-148 | Donkey-anti-guinea pig | 1:1000 | ||
| 705-096-147 | Donkey-anti-goat | 1:1000 | ||
| Biotinylated IgGs | E 0432 | Goat anti-rabbit | 1:1000 | DAKO, E 0432 |
| Biotin conjugated F(ab)’ fragment of affinity-purified IgG (H + L) | 711-1622 | Anti-rabbit | 1:1000 | BioTrend, 711-1622 |
| AMCA-conjugated IgG (H + L) | 715-155-151 | Donkey-anti-mouse | 1:50 | Jackson |
| 715-155-153 | Donkey-anti-rat | 1:50 | ||
| 705-156-147 | Donkey-anti-goat | 1:50 | ||
| CY3-conjugated streptavidin | 016-160-084 | - | 1:9000 | |