Table 2.
Neurochemical characterization of zinc-transporter-3-immunoreactive (ZnT3 +) neurons in the enteric ganglia of the porcine jejunum (MP myenteric plexus, OSP outer submucosal plexus, ISP inner submucosal plexus, s single neurons). Note that PGP9.5 is a pan-neuronal marker that marks all neurons in the tissue, and so PGP+/ZnT3+ cells illustrate the percentages of MP, OSP, and ISP neurons co-expressing ZnT3. In the triple-labeling studies, ZnT3-positive neurons were considered as representing 100% for all combinations with other neurotransmitters, and so all the values presented are percentages (means±SD) of ZnT3+ neurons
| Labeling | MP | OSP | ISP |
|---|---|---|---|
| PGP+/ZnT3+ | 38.6±4.0 | 48.7±10.8 | 44.8±4.1 |
| ZnT3+/VAChT− | 39.2±5.6 | 5.8±1.2 | 2.8±1.0 |
| ZnT3+/VAChT−/−NOS+ | 37.7±5.4 | 4.1±1.8 | 0 |
| ZnT3+/VAChT−/VIP+ | 5.8±3.4 | 1.3±0.9 | 2.1±0.9 |
| ZnT3+/VAChT−/SOM+ | 5.4±1.2 | 2.2±0.8 | 1.1±0.8 |
| ZnT3+/VAChT−/SP+ | 2.5±1.3 | 0.9±0.5 | 1.5±0.7 |
| ZnT3+/VAChT−/LENK+ | 1.2±0.6 | 0 | 0 |
| ZnT3+/LENK+/SP+ | s | 0 | 0 |
| ZnT3+/VAChT−/GAL+ | 0 | s | 2.0±0.8 |
| ZnT3+/VAChT−/NPY+ | s | 0 | 0 |
| ZnT3+/VAChT−/CGRP+ | 0 | 0 | 0 |
| ZnT3+/VAChT+ | 60.8±5.6 | 94.2±1.2 | 97.2±4.3 |
| ZnT3+/VAChT+/SOM+ | 2.2±1.2 | 41.1±1.8 | 27.2±5.4 |
| ZnT3+/VAChT+/VIP+ | 1.1±0.3 | 21.9±1.3 | 30.9±2.3 |
| ZnT3+/VAChT+/SP+ | 2.5±1.3 | 16.1±2.8 | 28.4±1.9 |
| ZnT3+/VAChT+/GAL+ | 0 | 0 | 47.1±1.9 |
| ZnT3+/VAChT+/NOS+ | 10.4±3.1 | 3.7±1.1 | 0 |