Figure 4.

Repo-Man^TA3 Is Sufficient to Trigger Deposition of Importin β and Nup153 on the Chromosomes Independently of Mitotic Exit

(A and B) DT40 cells transfected with GFP:Repo-Man^WT (A and B, 1–4) or GFP:Repo-Man^TA3 (green) (A and B, 5–8) and stained (red) for Importin β (A) or Nup153. Insets in (B) show a blowup of the indicated regions of enrichment for Repo-Man and Nup153.

(C) DT40 cells transfected with GFP:Repo-Man^WT (green) (1–4) or GFP:Repo-Man^TA3 (green) (5–12) and stained with antibodies (red) against MEL-28 (1–8) or LaminB1 (9–12).