Fig. 1.
PE is selectively depleted in inner membrane vesicles from psd1Δ and psd1Δ psd2Δ mitochondria. The S. cerevisiae strains crd1Δ, psd1Δ, and psd1Δ psd2Δ in the BY4741 background69 were grown in YPLac medium39 at 30 °C to early logarithmic growth phase. Mitochondria were isolated by differential centrifugation, the protein concentrations were adjusted, and inner membrane vesicles were isolated by sucrose gradient centrifugation as previously described.4,70 Phospholipids were extracted, separated by thin-layer chromatography, and analyzed as reported previously.69,71,72 The amounts of individual phospholipid classes were determined using a phosphate solution with 1 mg/ml phosphor as standard.73 Shown are the mean values of two determinations with range. DMPE, dimethylphosphatidylethanolamine; LP, lysophospholipids; PA, phosphatidic acid; PC, phosphatidylcholine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PS, phosphatidylserine.