Fig. 4.
Depletion of PE stabilizes respiratory chain supercomplexes. (a–c) Wild-type, psd1Δ, psd1Δ psd2Δ, and crd1Δ mitochondria were lysed with 1% (w/v) digitonin in digitonin buffer and subjected to blue native electrophoresis, followed by Western blotting using the indicated antisera. III, cytochrome bc1 complex; IV, cytochrome c oxidase; V2, ATP synthase dimer; V, ATP synthase monomer; Atp4, ATP synthase subunit 4 (subunit b); Cox4, cytochrome c oxidase subunit 4; Cox6, cytochrome c oxidase subunit 6; Rip1, Rieske iron–sulfur protein; SDH, succinate dehydrogenase (complex II).