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Multi-protein Complex Discovery. (A) The pull-down study using HaloTag recombinant RpoA. M: molecular weight marker; 1: unbound protein; 2: wash; 3: eluted protein after TEV protease cleavage, 4: eluted protein after removal of TEV protease; 5: concentrated protein sample. The arrows indicate the position of HaloTag recombinant (lane 1) and cleaved (lane 3) RpoA. (B) In-teraction map of E. coli proteins which identified by MALDI-MS/MS from the E. coli RpoA pull-down study.
