Figure 2.

EPR spectra of preloaded TEMPONE in T. latifolia pollen at different levels of dehydration and rehydration. Vapor-prehydrated pollen was incubated for 5 min in germination medium that was replaced by TEMPONE/ferricyanide. After 5 more min, the pollen was filtered and EPR spectra were recorded in the hydrated state (2.70 g water g⁻¹ DW) (a); after 2 h of exposure to laboratory air (0.80 g water g⁻¹ DW) (b); after 5 h of exposure to laboratory air (0.05 g water g⁻¹ DW) (c); and after forced rehydration of dried pollen in humid air (0.85 g water g⁻¹ DW) (d). The arrow in c points to the anisotropic spectrum, representing slow-moving TEMPONE molecules trapped in a solid environment. The dotted line represents the position of the lipid peak of the spectra.