Table 2.
Identification results of proteins differentially expressed in basal and after ω-3 PUFAs supplementation
| Spot | Protein name | ANOVA (p < 0.05) | Fold change | Protein expression level after ω-3 supplementation | UniProt ID | Entry name | Mascot score | Sequence coverage (%) | Number of Peptidic masses identified | Number of MS/MS identified | MW (KDa) | pI |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 837 |
Apolipoprotein A-I |
0.044 |
1.18 |
Up-regulated |
P02647 |
APOA1 |
87 |
42 |
10 |
1 |
30.8 |
5.56 |
| 997 |
Clusterin |
0.029 |
1.3 |
Up-regulated |
P10909 |
CLUS |
735 |
47 |
30 |
8 |
53 |
5.89 |
| 610 |
Fibrinogen β |
0.048 |
1.1 |
Up-regulated |
P02675 |
FIBB |
233 |
49 |
29 |
4 |
56.6 |
8.54 |
| 612 |
Fibrinogen β |
0.045 |
1.1 |
Up-regulated |
P02675 |
FIBB |
140 |
45 |
20 |
1 |
56.6 |
8.54 |
| 721* |
Clusterin |
0.02 |
1.4 |
Up-regulated |
P10909 |
CLUS |
519 |
23 |
|
11 |
53 |
5.89 |
| Apolipoprotein A-I |
0.02 |
1.4 |
Up-regulated |
P02647 |
APOA1 |
478 |
39 |
|
13 |
30.8 |
5.56 |
|
| Haptoglobin-related protein |
0.02 |
1.4 |
Up-regulated |
P00739 |
HPTR |
477 |
26 |
|
12 |
39.5 |
6.63 |
|
| 732 |
Haptoglobin-related protein |
0.015 |
1.37 |
Up-regulated |
P00739 |
HPTR |
79 |
31 |
14 |
4 |
39.5 |
6.63 |
| 965 |
Serum paraoxonase |
0.003 |
1.6 |
Up-regulated |
P27169 |
PON1 |
142 |
15 |
9 |
4 |
39.8 |
5.08 |
| 970 |
Serum paraoxonase |
0.003 |
1.97 |
Up-regulated |
P27169 |
PON1 |
411 |
56 |
24 |
8 |
39.8 |
5.08 |
| 973 |
Serum paraoxonase |
0.003 |
1.86 |
Up-regulated |
P27169 |
PON1 |
235 |
31 |
14 |
4 |
39.8 |
5.08 |
| 825* |
Ig kappa chain C region |
0.036 |
1.27 |
Up-regulated |
P01834 |
IGKC |
872 |
86 |
|
8 |
11.6 |
5.87 |
| Apolipoprotein A-I |
0.036 |
1.27 |
Up-regulated |
P02647 |
APOA1 |
397 |
52 |
|
15 |
30.8 |
5.56 |
|
| 578* |
α-1-antitrypsin |
0.001 |
0.74 |
Donw-regulated |
P01009 |
A1AT |
364 |
51 |
|
18 |
46.7 |
5.59 |
| 986* |
Complement C1r subcomponent |
0.018 |
0.85 |
Donw-regulated |
P00736 |
C1R |
1601 |
65 |
|
45 |
80.1 |
6.21 |
| 987* |
Serum Albumin |
0.032 |
0.85 |
Donw-regulated |
P02768 |
ALBU |
1114 |
75 |
|
46 |
69.3 |
6.3 |
| Ig mu Chain C region |
0.032 |
0.85 |
Donw-regulated |
P01871 |
IGHM |
987 |
57 |
|
25 |
49.3 |
6.8 |
|
| Complement C1r subcomponent |
0.032 |
0.85 |
Donw-regulated |
P00736 |
C1R |
784 |
46 |
|
28 |
80.1 |
6.2 |
|
| 324 |
Complement Factor H |
0.017 |
0.72 |
Donw-regulated |
P08603 |
CFAH |
72 |
16 |
19 |
1 |
14.4 |
6.21 |
| 980* |
Fibronectin |
0.01 |
0.66 |
Donw-regulated |
P02751 |
FN1 |
1434 |
26 |
|
40 |
262.5 |
5.71 |
| 981* | Fibronectin | 0.002 | 0.67 | Donw-regulated | P02751 | FN1 | 1437 | 30 | 48 | 262.5 | 5.71 |
Spots label with a * denotes identification by Orbitrap. Positive fold changes denote proteins up-regulated while negative fold changes denote decrease protein expression after the supplementation. MW denotes Molecular Weight. pI denotes Isoelectric point.