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Representative images of tumor spheres from OVCAR3, TOV112D, and SKOV3 cells in a sphere culture system with serum-free DMEM-F12 (Invitrogen, Carlsbad, Calif., USA) supplemented with 10 ng/mL basic fibroblast growth factor (bFGF) and 20 ng/mL epidermal growth factor (EGF) and plated in an ultra-low attachment plate.
