Table 2.

Validation of miRNA microarray results using quantitative RT-PCR: miRNAs differentially expressed between OVCAR3 cells from the CD133⁺ sphere-forming subpopulation compared with cells cultured in a conventional adherent environment

	MicroRNA microarray		qRT-PCR
	Fold change	Pvalue	Fold change (mean ± SD)
has-miR-205	11.78	0.001	11.76 ± 1.32
has-miR-146a	7.33	<0.001	5.19 ± 4.14
has-miR-200a	2.45	0.0265	2.21 ± 2.54
has-miR-200b	2.13	0.014	1.81 ± 0.54
has-miR-31	2.35	0.024	2.45 ± 1.59
has-miR-1202	0.41	0.013	0.58 ± 0.22
has-miR-1181	0.47	0.023	0.67 ± 0.28