Capillary assays in cheD genetic backgrounds.
A. Capillary assays towards asparagine with mcpB expressed in the Δ10mcp background (■) and the Δ10mcp cheD background (). The Δ10mcp strain () is included as a negative control.
B. Capillary assays towards proline with mcpC expressed in the Δ10mcp background (■), mcpC expressed in the Δ10mcp cheD background (), mcpC-Q609E expressed in the Δ10mcp background (▼) and mcpC-Q609E expressed in the Δ10mcp cheD background (). The Δ10mcp strain () is included as a negative control.
C. Capillary assays towards proline with all other nine chemoreceptors present. Wild-type strain OI1085 (■), mcpC expressed in the cheD mcpC knockout (), mcpC-Q609E expressed in the mcpC knockout (▼) and mcpC-Q609E expressed in the cheD mcpC knockout ().